PNP antibody (Middle Region)

Catalog No. ABIN4886701

Product Details anti-PNP Antibody

Target: PNP (NP) (Nucleoside Phosphorylase (NP))

Binding Specificity: AA 161-189, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Purine nucleoside phosphorylase(PNP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: AMSDAYDRTM RQRALSTWKQ MGEQRELQE

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Purine nucleoside phosphorylase(PNP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: purine nucleoside phosphorylase
Protein Name: Purine nucleoside phosphorylase

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PNP (161-189aa AMSDAYDRTMRQRALSTWKQMGEQRELQE), different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for PNP

Target: PNP (NP) (Nucleoside Phosphorylase (NP))

Alternative Name: PNP (NP Products)

Synonyms: NP antibody, PRO1837 antibody, PUNP antibody, Np antibody, AL024301 antibody, AU015798 antibody, Np-1 antibody, Np-2 antibody, Pnp1 antibody, purine nucleoside phosphorylase antibody, purine-nucleoside phosphorylase antibody, PNP antibody, Pnp antibody

Target Type: Viral Protein

Background: The PNP gene encodes purine nucleoside phosphorylase, an enzyme that catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. It is presented results from gene dosage studies consistent with assignment of the PNP locus to band 14q13. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T-lymphocyte function and neurologic dysfunction.

Synonyms: Inosine phosphorylase | NP | Np1 | Nucleoside phosphorylase | PNP | Pnp1 | PRO1837 | PUNP | P00491

Gene ID: 4860

UniProt: P00491

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Ribonucleoside Biosynthetic Process, Positive Regulation of Response to DNA Damage Stimulus