FGF1 antibody (AA 16-155)

Catalog No. ABIN4886581

Product Details anti-FGF1 Antibody

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Binding Specificity: AA 16-155

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Fibroblast growth factor 1(FGF1) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Fibroblast growth factor 1(FGF1) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Gene Name: fibroblast growth factor 1(acidic)
Protein Name: Fibroblast growth factor 1

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human FGF1 recombinant protein (Position: F16-D155). Human FGF1 shares 96.4% amino acid (aa) sequence identity with both mouse and rat FGF1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-FGF1 antibody (ABIN4886581)

Zhang, Wang, Wang, Jiang, Yu, Chong, Fu: "Ferulic acid ameliorates nerve injury induced by cerebral ischemia in rats." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 972-976, (2015) (PubMed).

Gu, Zhang, Wu, Xu, Cui, Yang, Zhao, Sun: "Differential expression of decorin, EGFR and cyclin D1 during mammary gland carcinogenesis in TA2 mice with spontaneous breast cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 6, (2010) (PubMed).

Target Details for FGF1

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: FGF1 (FGF1 Products)

Synonyms: AFGF antibody, ECGF antibody, ECGF-beta antibody, ECGFA antibody, ECGFB antibody, FGF-1 antibody, FGF-alpha antibody, FGFA antibody, GLIO703 antibody, HBGF-1 antibody, HBGF1 antibody, Fgf1 antibody, FGF1 antibody, aFGF antibody, ecgf antibody, fgfa antibody, ecgfa antibody, ecgfb antibody, fgf-1 antibody, hbgf1 antibody, glio703 antibody, ecgf-beta antibody, fgf-alpha antibody, Dffrx antibody, Fam antibody, Fgf-1 antibody, Fgfa antibody, zgc:136885 antibody, LOC100221393 antibody, fgf1 antibody, zgc:73249 antibody, fibroblast growth factor 1 antibody, fibroblast growth factor 1 L homeolog antibody, fibroblast growth factor 1b antibody, fibroblast growth factor 1a antibody, FGF1 antibody, fgf1.L antibody, fgf1 antibody, Fgf1 antibody, fgf1b antibody, LOC100221393 antibody, fgf1a antibody

Background: Fibroblast growth factor 1 (acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It also acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.

Synonyms: ECGF | aFGF | FGF 1 | FGF-1 | FGF1 | Fgf-1 | Fgfa | FGFA | HBGF1 | HBGF-1 | fgf19 | P05230

Gene ID: 2246

UniProt: P05230

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway