CPB2 antibody (AA 166-388)

Catalog No. ABIN4886545

Product Details anti-CPB2 Antibody

Target: CPB2 (Carboxypeptidase B2 (Plasma) (CPB2))

Binding Specificity: AA 166-388

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Carboxypeptidase B2(CPB2) detection. Tested with WB, IHC-P, ELISA in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Carboxypeptidase B2(CPB2) detection. Tested with WB, IHC-P, ELISA in Human.
Gene Name: carboxypeptidase B2
Protein Name: Carboxypeptidase B2

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CPB2 recombinant protein (Position: K166-D388). Human CPB2 shares 86.1% and 85.7% amino acid (aa) sequence identity with mouse and rat CPB2, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CPB2 antibody (ABIN4886545)

Qin, Zeng, Tian, Deng, Ren, Zheng, Li, Li, Liao, Chen: "Involvement of prolylcarboxypeptidase in the effect of rutaecarpine on the regression of mesenteric artery hypertrophy in renovascular hypertensive rats." in: Clinical and experimental pharmacology & physiology, Vol. 36, Issue 3, pp. 319-24, (2009) (PubMed).

Target Details for CPB2

Target: CPB2 (Carboxypeptidase B2 (Plasma) (CPB2))

Alternative Name: CPB2 (CPB2 Products)

Synonyms: CPU antibody, PCPB antibody, TAFI antibody, 1110032P04Rik antibody, 4930405E17Rik antibody, AI255929 antibody, CPR antibody, Cpu antibody, pCPB antibody, zgc:112493 antibody, carboxypeptidase B2 antibody, carboxypeptidase B2 (plasma) antibody, CPB2 antibody, Cpb2 antibody, cpb2 antibody

Background: Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Synonyms: Carboxypeptidase U | CarboxypeptidaseU | CarboxypeptidaseB2 | Carboxypeptidase B2 | CP B2 | CPB2 | CPB 2 | CPU | PCPB | Procarboxypeptidase B | Procarboxypeptidase R | Procarboxypeptidase U | TAFI | Q96IY4

Gene ID: 1361

Pathways: Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis