CD33 antibody (AA 18-259)
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- Target See all CD33 Antibodies
- CD33
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Binding Specificity
- AA 18-259
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CD33 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Myeloid cell surface antigen CD33(CD33) detection. Tested with WB, IHC-P, FCM in Human.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Myeloid cell surface antigen CD33(CD33) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: CD33 Molecule
Protein Name: Myeloid cell surface antigen CD33 - Purification
- Immunogen affinity purified.
- Immunogen
- E. coli-derived human CD33 recombinant protein (Position: D18-H259). Human CD33 shares 61.6% amino acid (aa) sequence identity with mouse CD33.
- Isotype
- IgG
- Top Product
- Discover our top product CD33 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- CD33
- Alternative Name
- CD33 (CD33 Products)
- Synonyms
- SIGLEC-3 antibody, SIGLEC3 antibody, p67 antibody, Siglec-3 antibody, gp67 antibody, CD33 molecule antibody, CD33 antigen antibody, CD33 antibody, Cd33 antibody
- Background
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CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
Synonyms: CD 33 | CD33 | CD33 antigen (gp67) | CD33 antigen | CD33 Molecule | gp67 | My9 | p67 | SIGLEC 3 | SIGLEC3 | Siglec-3 | P20138 - Gene ID
- 945
- UniProt
- P20138
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