CCT5 antibody (AA 443-535)

Catalog No. ABIN4886515

Product Details anti-CCT5 Antibody

Target: CCT5 (Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5))

Binding Specificity: AA 443-535

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for T-complex protein 1 subunit epsilon(CCT5) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for T-complex protein 1 subunit epsilon(CCT5) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: chaperonin containing TCP1 subunit 5
Protein Name: T-complex protein 1 subunit epsilon

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human TCP1 epsilon recombinant protein (Position: L443-K535). Human TCP1 epsilon shares 95.7% amino acid (aa) sequence identity with both mouse and rat TCP1 epsilon.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CCT5

Target: CCT5 (Chaperonin Containing TCP1, Subunit 5 (Epsilon) (CCT5))

Alternative Name: CCT5 (CCT5 Products)

Synonyms: CCT-epsilon antibody, CCTE antibody, TCP-1-epsilon antibody, wu:fb54h08 antibody, CCT5 antibody, Cct5 antibody, DKFZp469K0732 antibody, cct5a antibody, Ccte antibody, TCPE antibody, mKIAA0098 antibody, chaperonin containing TCP1 subunit 5 antibody, chaperonin containing TCP1, subunit 5 (epsilon) antibody, chaperonin containing TCP1 subunit 5 S homeolog antibody, Chaperonin Containing TCP-1 antibody, T-complex protein 1 subunit epsilon antibody, chaperonin containing Tcp1, subunit 5 (epsilon) antibody, chaperonin containing TCP1, subunit 5 (epsilon) b antibody, CCT5 antibody, Cct5 antibody, cct5 antibody, cct5.S antibody, cct-5 antibody, LOC100632800 antibody, cct5b antibody

Background: CCT5 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13.

Synonyms: CCT5 | CCT 5 | CCTepsilon | CCT epsilon | TCP 1 epsilon | TCP1epsilon | TCPE | P48643

Gene ID: 22948

UniProt: P48643