CA2 antibody (AA 2-260)

Catalog No. ABIN4886493

Product Details anti-CA2 Antibody

Target: CA2 (Carbonic Anhydrase II (CA2))

Binding Specificity: AA 2-260

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Carbonic anhydrase 2(CA2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Carbonic anhydrase 2(CA2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: carbonic anhydrase II
Protein Name: Carbonic anhydrase 2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CA2 recombinant protein (Position: S2-K260). Human CA2 shares 81.1% and 80.7% amino acid (aa) sequence identity with mouse and rat CA2, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: CA2 (CA2 Products)

Synonyms: CG6906 antibody, CT21171 antibody, Dmel\\CG6906 antibody, CA-2 antibody, caII antibody, car2 antibody, ca ii antibody, ca-ii antibody, BETA CA2 antibody, BETA CARBONIC ANHYDRASE 2 antibody, CA18 antibody, CARBONIC ANHYDRASE 18 antibody, CARBONIC ANHYDRASE 2 antibody, T9L3.40 antibody, T9L3_40 antibody, carbonic anhydrase 2 antibody, LOC100135827 antibody, AI131712 antibody, CAII antibody, Ca2 antibody, Car-2 antibody, Ltw-5 antibody, Lvtw-5 antibody, CA-II antibody, CAC antibody, Car2 antibody, CAR3 antibody, Carbonic anhydrase 2 antibody, carbonic anhydrase 2 L homeolog antibody, carbonic anhydrase 2 antibody, carbonic anhydrase 1 antibody, carbonic anhydrase II antibody, carbonic anhydrase, alpha type, periplasmic antibody, CAH2 antibody, ca2.L antibody, ca2 antibody, CA2 antibody, HCAG_04637 antibody, LOC100135827 antibody, LOC100635510 antibody, Car2 antibody, Ca2 antibody, LOC100154873 antibody, LOC101113341 antibody

Background: CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.

Synonyms: 4.2.1.1 | CA2 | CAC | CAH2 | CAII | CA-II | Carbonate dehydratase II | Carbonic anhydrase 2 | Carbonic anhydrase B | Carbonic anhydrase C | Carbonic Anhydrase II | Carbonic dehydratase | P00918

Gene ID: 760

UniProt: P00918