ADAR antibody (AA 128-346)

Catalog No. ABIN4886426

Product Details anti-ADAR Antibody

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Binding Specificity: AA 128-346

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Double-stranded RNA-specific adenosine deaminase(ADAR) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Double-stranded RNA-specific adenosine deaminase(ADAR) detection. Tested with WB, IHC-P in Human.
Gene Name: adenosine deaminase, RNA-specific
Protein Name: Double-stranded RNA-specific adenosine deaminase

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ADAR1 recombinant protein (Position: S128-Q346). Human ADAR1 shares 90.2% and 50.7% amino acid (aa) sequence identity with mouse and rat ADAR1, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ADAR

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Alternative Name: ADAR (ADAR Products)

Synonyms: red1 antibody, drada antibody, wu:fc22a02 antibody, adar1 antibody, dsRAD antibody, dsRAD-1 antibody, ADAR antibody, ADAR1 antibody, CG12598 antibody, Dmel\\CG12598 antibody, EG:BACN35H14.1 antibody, adar antibody, adr antibody, cg12598 antibody, dADAR antibody, dAdar antibody, hypnos-2 antibody, NV18763 antibody, AGS6 antibody, DRADA antibody, DSH antibody, DSRAD antibody, G1P1 antibody, IFI-4 antibody, IFI4 antibody, K88DSRBP antibody, P136 antibody, AV242451 antibody, Adar1 antibody, mZaADAR antibody, adenosine deaminase, RNA-specific antibody, adenosine deaminase, RNA-specific S homeolog antibody, adenosine deaminase, RNA specific antibody, Adenosine deaminase acting on RNA antibody, adenosine deaminase acting on RNA antibody, double-stranded RNA-specific editase 1 antibody, adar antibody, adar.S antibody, ADAR antibody, Adar antibody, CpipJ_CPIJ011849 antibody, LOC100114127 antibody

Background: Double-stranded RNA-specific adenosine deaminase, also known as ADAR1, is an enzyme that in humans is encoded by the ADAR gene. It is mapped to 1q21.3. This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.

Synonyms: Adar1 | Adar 1 | ADAR | AGS6 | AV242451 | DRADA | Dsh | Dsrad | G1P1 | IFI4 | IFI-4 | IFI 4 | P136 | P55265

Gene ID: 103

UniProt: P55265

Pathways: Protein targeting to Nucleus