ACSL1 antibody (AA 604-698)

Catalog No. ABIN4886418

Product Details anti-ACSL1 Antibody

Target: ACSL1 (Acsl1) (Acyl-CoA Synthetase Long-Chain Family Member 1 (Acsl1))

Binding Specificity: AA 604-698

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACSL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Long-chain-fatty-acid--CoA ligase 1(ACSL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Long-chain-fatty-acid--CoA ligase 1(ACSL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: acyl-CoA synthetase long-chain family member 1
Protein Name: Long-chain-fatty-acid--CoA ligase 1

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ACSL1 recombinant protein (Position: D604-V698). Human ACSL1 shares 81.1% and 86.3% amino acid (aa) sequence identity with mouse and rat ACSL1, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ACSL1

Target: ACSL1 (Acsl1) (Acyl-CoA Synthetase Long-Chain Family Member 1 (Acsl1))

Alternative Name: ACSL1 (Acsl1 Products)

Synonyms: ACS1 antibody, FACL1 antibody, FACL2 antibody, LACS antibody, LACS1 antibody, LACS2 antibody, Acas antibody, Acas1 antibody, Acs antibody, FACS antibody, Facl2 antibody, ACS antibody, COAA antibody, zgc:110081 antibody, CER8 antibody, ECERIFERUM 8 antibody, LONG-CHAIN ACYL-COA SYNTHASE 1 antibody, T8I13.8 antibody, F13F21.14 antibody, F13F21_14 antibody, LATERAL ROOT DEVELOPMENT 2 antibody, LRD2 antibody, long-chain acyl-CoA synthetase 2 antibody, acyl-CoA synthetase long chain family member 1 antibody, acyl-CoA synthetase long-chain family member 1 antibody, acyl-CoA synthetase long chain family member 1a antibody, AMP-dependent synthetase and ligase family protein antibody, long-chain acyl-CoA synthetase 2 antibody, ACSL1 antibody, Acsl1 antibody, acsl1a antibody, LACS1 antibody, LACS2 antibody

Background: Long-chain-fatty-acid-CoA ligase 1 is an enzyme that in humans is encoded by the ACSL1 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. This specific protein is most commonly found in mitochondria and peroxisomes.

Synonyms: ACS 1 | ACS1 | ACSL 1 | ACSL1 | FACL 1 | FACL1 | FACL 2 | FACL2 | LACS 1 | LACS1 | LACS 2 | LACS2 | P33121

Gene ID: 2180

UniProt: P33121

Pathways: Regulation of Lipid Metabolism by PPARalpha