Urease antibody (Biotin)
URE
Reactivity: Canavalia ensiformis
WB, ELISA, IF, IHC
Host: Rabbit
Polyclonal
Biotin
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- Target See all Urease (URE) products
- Urease (URE)
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Reactivity
- Canavalia ensiformis
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Urease antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
- Characteristics
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Expected / apparent Molecular Weight of the Antigene: 91 kDa
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radialimmunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition. - Purification
- affinity purified
- Immunogen
- urease isolated and purified from Canavalia ensiformis (common jack-beans)
- Isotype
- IgG
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anti-Urease (URE) antibody
URE Reactivity: Jack Bean WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Recommended Dilution: 1 : 1000 - 1 : 30 000 for techniques listed above.
- Comment
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Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution please add 1 mL of sterile destilled water.
- Buffer
- phosphate buffer saline
- Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- Storage
- -20 °C
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- Target
- Urease (URE)
- Alternative Name
- Urease (URE Products)
- Synonyms
- F12B7.10 antibody, F12B7_10 antibody, urease antibody, urease antibody, URE antibody, CNH01900 antibody
- Background
- Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
- Molecular Weight
- 91 kDa
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