Lhcb1 antibody

Catalog No. ABIN488535

Product Details anti-Lhcb1 Antibody

Target: Lhcb1 (lhcb1) (Chlorophyll a-b binding protein (lhcb1))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lhcb1 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radialimmunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Purification: serum

Immunogen: BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At1g34430 (Lhcb1.4), and At1g34420 (Lhcb1.5)

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 2500 - 1 : 5000 with standard ECL (WB).

Comment:

This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL of sterile water

Buffer: PBS pH 7.4

Handling Advice: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

References for anti-Lhcb1 antibody (ABIN488535)

Yoshida, Watanabe, Hachiya, Tholen, Shibata, Terashima, Noguchi: "Distinct responses of the mitochondrial respiratory chain to long- and short-term high-light environments in Arabidopsis thaliana." in: Plant, cell & environment, (2011) (PubMed).

Target Details for Lhcb1

Target: Lhcb1 (lhcb1) (Chlorophyll a-b binding protein (lhcb1))

Alternative Name: Lhcb1 (lhcb1 Products)

Synonyms: Cab-m7 antibody, GRMZM2G402936 antibody, LHCP antibody, light harvesting complex mesophyll 7 antibody, lhcb7 antibody

Background: AGI Code: At1g29910, At1g29920, At1g29930, At1g34420, At1g34430
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.

Molecular Weight: expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana

UniProt: P04777, Q8VZ87, P04778, Q9C5R6, Q39141