SELPLG antibody (FITC)

Catalog No. ABIN488144

Product Details anti-SELPLG Antibody (FITC)

Target: SELPLG (Selectin P Ligand (SELPLG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SELPLG antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with Human PSGL-1 antigen.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein-A Sepharose Chromatography.

Immunogen: Human Neutrophil PSGL-1. Remarks: This Hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8-653 with Balb/c mouse splenocyte.

Clone: PL2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10-20 μg/mL (final concentration). Positive Controls: Jurkat, HL60.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric Analysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 µL of normal goat serum to the cell pellet aftertapping. Mix well, and incubate for 5 minutes at roomtemperature (20~25°C). 3) Add 30 µL of the FITC labeled CD162 (PL2) monoclonal antibody (10-20 µg/mL) dilutedwith the washing buffer. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD162 (PL1) monoclonal antibody (50 µg/mL)into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C)5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.05 mg/mL

Buffer: PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for SELPLG

Target: SELPLG (Selectin P Ligand (SELPLG))

Alternative Name: CD162 / PSGL1 (SELPLG Products)

Background: CD162 is a 120kD molecule also known as P-Selectin Glycoprotein Ligand I (PSGL-1). CD162 is the major counter receptor for P-selectin and is involved in neutrophil attachment and rolling to endothelium. In peripheral blood CD162 is expressed by monocytes, granulocytes, T cells and some B cells.Synonyms: P-selectin glycoprotein ligand 1, PSGL-1, SELPLG, Selectin P ligand

Gene ID: 6404

NCBI Accession: NP_002997

UniProt: Q14242