CRYbA4 antibody (N-Term)

Catalog No. ABIN488078

Product Details anti-CRYbA4 Antibody

Target: CRYbA4 (Crystallin, beta A4 (CRYbA4))

Binding Specificity: AA 1-185, N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYbA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Recognizes Beta-crystallin A4 (1-150).

Cross-Reactivity (Details): Species reactivity (expected):Mouse (91 %), Rat (91 %), Bovine (94 %), Dog (94 %), Monkey (96 %).
Species reactivity (tested):Human, Mouse.

Purification: Antigen-Affinity Chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 185 of Beta-Crystallin A4 Genename: CRYBA4

Application Details

Application Notes: This antibody is suitable for use in Immunofluorescence/Immunocytochemistry andWestern blotting. Recommended Dilutions: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 10 % Glycerol and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for CRYbA4

Target: CRYbA4 (Crystallin, beta A4 (CRYbA4))

Alternative Name: beta-Crystallin A4 (CRYbA4 Products)

Synonyms: zgc:109979 antibody, CRYBA4 antibody, CTRCT23 antibody, MCOPCT4 antibody, crystallin beta A4 antibody, crystallin, beta A4 antibody, CRYBA4 antibody, cryba4 antibody, Cryba4 antibody

Background: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families, beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3.Synonyms: Beta-A4 crystallin, CRYBA4

Molecular Weight: 22 kDa

Gene ID: 1413, 9606

UniProt: P53673