MLL4 antibody (C-Term)

Catalog No. ABIN488001

Product Details anti-MLL4 Antibody

Target: MLL4 (Histone-Lysine N-Methyltransferase MLL4 (MLL4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This MLL4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to MLL4.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence CNCGAKRCRRFLN, from the C Terminus of the protein sequence according to NP_055542.

Application Details

Application Notes: Peptide ELISA: 1/128000. Western blot: Preliminary experiments gave no signal but low background in Human Heartlysates at up to 1 μg/mL. However this used our routine western blotting protocol which wewould not expect to reliably detect proteins as large as the predicted size of approx.312KDa according to NP_055542. Immunohistochemistry on paraffin sections: Human Testis shows cytoplasm staining ofspermatids. Recommended concentration: 5 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MLL4

Target: MLL4 (Histone-Lysine N-Methyltransferase MLL4 (MLL4))

Alternative Name: MLL4 (MLL4 Products)

Synonyms: HRX2 antibody, MLL1B antibody, MLL2 antibody, MLL4 antibody, TRX2 antibody, WBP7 antibody, 2610014H22Rik antibody, Mll2 antibody, Wbp7 antibody, mKIAA0304 antibody, ALR antibody, BC032281 antibody, BC058659 antibody, C430014K11Rik antibody, KMT2B antibody, Mll4 antibody, RGD1308331 antibody, lysine methyltransferase 2B antibody, lysine (K)-specific methyltransferase 2B antibody, lysine (K)-specific methyltransferase 2D antibody, KMT2B antibody, Kmt2b antibody, Kmt2d antibody

Background: KMT2D / MLL4 contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This protein is ubiquitously expressed in adult tissues where it possibly acts as a transcriptional regulatory factor. It is also expressed in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced gene transcripts encoding distinct isoforms have been reported, however, the full length nature of the shorter transcript is not known.Synonyms: HRX2, Histone-lysine N-methyltransferase MLL4, KIAA0304, KMT2D, Lysine N-methyltransferase 2D, MLL2, Myeloid/lymphoid or mixed-lineage leukemia protein 4, TRX2, Trithorax homolog 2, WBP7, WW domain-binding protein 7

Gene ID: 9757

NCBI Accession: NP_055542

UniProt: Q9UMN6

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Warburg Effect