MORF4L1 antibody (N-Term)

Catalog No. ABIN487997

Product Details anti-MORF4L1 Antibody

Target: MORF4L1 (Mortality Factor 4 Like 1 (MORF4L1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This MORF4L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: APKQDPKPKF QEG-C

Specificity: This antibody recognizes MRG15 and is expected to recognise Isoform 1 (NP_006782.1) and Isoform 2 (NP_996670.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Syntehtic peptide from the N-Terminus of Human MORF4L1 (NP_006782.1, NP_996670.1) . Genename: MORF4L1

Application Details

Application Notes: Peptide ELISA: 1/64000. Western blot: Preliminary experiments gave an approx 40 kDa band in Human Liver lysatesand an approx 100 kDa band in Human Heart lysates at 1 μg/mL. Please note that currentlywe cannot find an explanation in the literature for the bands we observe given thecalculated size of 37.0 kDda according to NP_006782.1. Immunohistochemistry on paraffin sections: Human Uterus shows nuclear staining in theepithelial cells of the endometrial glands. Recommended concentration: 2 - 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MORF4L1

Target: MORF4L1 (Mortality Factor 4 Like 1 (MORF4L1))

Alternative Name: MRG15 (MORF4L1 Products)

Background: MRG15 ia a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis and DNA repair.Synonyms: FWP006, HSPC008, HSPC061, MORF-related gene 15 protein, MORF4L1, MRG15, MSL3-1 protein, Mortality factor 4-like protein 1, PP368, Transcription factor-like protein MRG15

Molecular Weight: 37.2 kDa (Mouse NP_077751.1).

Gene ID: 10933

NCBI Accession: NP_006782

UniProt: Q9UBU8

Pathways: Chromatin Binding