TJP1 antibody (Internal Region)

Catalog No. ABIN487963

Product Details anti-TJP1 Antibody

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to zonula occludens 1 protein.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-PKPTSQNQFSEHDKT, from the internal region of the protein sequence according to NP_003248.3, NP_783297.2.

Application Details

Application Notes: Peptide ELISA: 1/128000. Western blot: 0.3 - 1.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: ZO-1 / Tight Junction Protein 1 (TJP1 Products)

Background: Zona Occludens or tight junctions are plasma membrane structures that seal together the perimeters of polarized epithelial cell monolayers and provide the cellular barrier necessary to maintain vectoral secretion, absorption and transport, often defining luminal and intracellular space. Four tight junction specific proteins have been identified (ZO1, ZO2, ZO3 and occludin). ZO1 was the first protein shown to be specifically associated with the tight junction and remains the standard marker for these structures.Synonyms: Tjp1, Zo1, Zona occludens protein 1, Zonula occludens protein 1

Gene ID: 7082

NCBI Accession: NP_003248

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization