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Methotrexate antibody

MTX EIA Host: Sheep Polyclonal unconjugated
Catalog No. ABIN487772
  • Target See all Methotrexate (MTX) Antibodies
    Methotrexate (MTX)
    Reactivity
    Please inquire
    Host
    • 3
    Sheep
    Clonality
    • 2
    Polyclonal
    Conjugate
    • 3
    This Methotrexate antibody is un-conjugated
    Application
    • 3
    • 1
    • 1
    Enzyme Immunoassay (EIA)
    Purification
    Ig Fraction
    Immunogen
    Methotrexate -BSA
    Isotype
    IgG
    Top Product
    Discover our top product MTX Primary Antibody
  • Application Notes
    ELISA: 2.5 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    7.74 mg/mL (U.V.abs at 280nm)
    Buffer
    20 mM Phosphate, 150 mM Sodium Chloride, pH 7.2 containing 0.09 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
  • Target
    Methotrexate (MTX)
    Alternative Name
    Methotrexate (MTX Products)
    Target Type
    Amino Acid
    Background
    Methotrexate (rINN) abbreviated MTX and formerly known as amethopterin, is an antimetabolite and antifolate drug used in treatment of cancer, autoimmune diseases and as an abortifacient in the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Methotrexate competitively inhibits dihydrofolate reductase (DHFR), an enzyme that participates in the tetrahydrofolate synthesis. The affinity of methotrexate for DHFR is about one thousand-fold that of folate for DHFR. Methotrexate acts specifically during DNA and RNA synthesis, and thus it is cytotoxic during the S-phase of the cell cycle. Logically, it therefore has a greater toxic effect on rapidly dividing cells which replicate their DNA more frequently, and thus inhibits the growth and proliferation of these non-cancerous cells. Lower doses of methotrexate have been shown to be very effective for the management of rheumatoid arthritis, Crohn's disease, and psoriasis.Synonyms: 4-amino-10-methylpteroyl-L-glutamic acid
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