Kanamycin antibody

Catalog No. ABIN487712

Product Details anti-Kanamycin Antibody

Target: Kanamycin

Reactivity: Streptomyces kanamyceticus

Host: Sheep

Clonality: Polyclonal

Conjugate: This Kanamycin antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Purification: Ig Fraction

Immunogen: Kanamycin-BTG

Isotype: IgG

Application Details

Application Notes: ELISA: 0.312 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10.77 mg/mL (by U.V. abs at 280nm)

Buffer: 20 mM Phosphate, 150 mM Sodium Chloride, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at-20 °C.

Target Details for Kanamycin

Target: Kanamycin

Abstract: Kanamycin Products

Target Type: Chemical

Background: Kanamycin (also known as kanamycin A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a wide variety of infections. Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is kanamycin sulfate. Kanamycin is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for kanamycin resistance (primarily Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin (50-100 μg/mL) containing agar plates or are grown in media containing kanamycin (50-100 μg/mL). Only the bacteria that have successfully taken up the kanamycin resistance gene become resistant and will grow under these conditions. As a powder kanamycin is white to off-white and is soluble in water (50 mg/mL). Mammalian cells and other eukaryotes are screened using G418, a similar aminoglycoside antibiotic, which KanMX confers resistance against. At least one such gene, Atwbc19 is native to a plant species, of comparatively large size and its coded protein acts in a manner which decreases the possibility of horizontal gene transfer from the plant to bacteria, it may be incapable of giving resistance to kanamycin to bacteria even if gene transfer occurs.Synonyms: Kanamycin A, Kantrex