PAK1/2/3 antibody (pThr423)

Catalog No. ABIN479638

Product Details anti-PAK1/2/3 Antibody

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Binding Specificity: pThr423

Reactivity: Human, Rat, Mouse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1/2/3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Detects endogenous human PAK1, PAK2 and PAK3 only when phosphorylated at Thr423, Thr402 and Thr421, respectively. Does not cross-react with phosphorylated PAK4, PAK5 or PAK6. Does cross-react with phospho-Mst1 (Thr183) or phospho-Mst2 (Thr180). Species cross-reactivity: Guinea pig. Predicted to cross-react with mouse and rat based on sequence homology.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr423 of human PAK1.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:1000)

Usage: Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK1 + PAK2 + PAK3 (PAK1/2/3 Products)

Background: Name/Gene ID: PAK1/PAK2/PAK3

Synonyms: PAK1/PAK2/PAK3

This antibody detects: PAK1 + PAK2 + PAK3