PAK1/2 antibody (pSer144, Ser141)
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- Target See all PAK1/2 products
- PAK1/2 (P21 Protein (Cdc42/Rac)-Activated Kinase 1/2 (PAK1/2))
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Binding Specificity
- pSer144, Ser141
- Reactivity
- Human, Rat, Mouse, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PAK1/2 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Recognizes Ser144 phosphorylated PAK1 and Ser141 phosphorylated PAK2. This antibody also recognizes Ser139 phosphorylated PAK3 but does not cross-react with phosphorylated PAK4-6. The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase and growth factor-induced neurite outgrowth. Species cross-reactivity: Human and guinea pig, predicted to react with rat and mouse due to 100 % sequence homology.
- Purification
- Protein A purified
- Immunogen
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KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser144 of human PAK1.
Type of Immunogen: Synthetic phospho-peptide - Isotype
- IgG
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- Application Notes
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Approved: WB (1:1000)
Usage: Suitable for use in Western Blot. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.
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- Target
- PAK1/2 (P21 Protein (Cdc42/Rac)-Activated Kinase 1/2 (PAK1/2))
- Alternative Name
- PAK1/PAK2 (PAK1/2 Products)
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