PAK1/2 antibody (pSer199, Ser204)
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- Target See all PAK1/2 products
- PAK1/2 (P21 Protein (Cdc42/Rac)-Activated Kinase 1/2 (PAK1/2))
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Binding Specificity
- pSer199, Ser204
- Reactivity
- Human, Rat, Mouse, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PAK1/2 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- Detects endogenous levels of Ser199/204 phosphorylated human PAK1 or Ser192/197 phosphorylated human PAK2. It may also detect Ser200/205 phosphorylated PAK3, however it does not cross-react with phosphorylated PAK4. Species cross-reactivity: Guinea pig. Predicted to cross-react with mouse and rat based on sequence homology.
- Purification
- Protein A purified
- Immunogen
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser199/204 of human PAK1.
Type of Immunogen: Synthetic phospho-peptide - Isotype
- IgG
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- Application Notes
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Approved: WB (1:1000)
Usage: Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
- Preservative
- Without preservative
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- Target
- PAK1/2 (P21 Protein (Cdc42/Rac)-Activated Kinase 1/2 (PAK1/2))
- Alternative Name
- PAK1/PAK2 (PAK1/2 Products)
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