Nuclei and Chromosomes, Histone Specific antibody
Reactivity: Human
ELISA, IF, IHC, IHC (p), ICC
Host: Mouse
Monoclonal
unconjugated
1 image
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- Target
- Nuclei and Chromosomes, Histone Specific
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Specificity
- Histone specific, stains nuclei and chromosomes of cells of all species including animals and plants. Reacts preferentially with Histone H1 by ELISA and immunoblot. Produces a speckled nuclear staining pattern in all tissues studies. Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Nuclear staining in interphase cells is intense and diffuse.
- Purification
- Ammonium sulfate precipitation
- Immunogen
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Type of Immunogen: Native protein - Isotype
- IgG2a
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- Application Notes
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Approved: ELISA (1 - 4 μg/mL), ICC (1:20 - 1:30), IF, IHC, IHC-P (1:50)
Usage: Suitable for use in ELISA, Immunocytochemistry, Indirect Immunofluorescence and Immunohistochemistry. Immunocytochemistry (Indirect immunofluorescence): 1:20-1:30, 30-100 μL per slide/well using paraformaldehyde/acetone fixed cell preparation. Immunohistochemistry: Formalin fixed, paraffin embedded tissue sections or paraformaldehyde/acetone fixed frozen tissue sections. Positive control: Human tonsil. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Nuclei and Chromosomes, Histone Specific
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