H-2Dd antibody (N-Term) (PE)

Catalog No. ABIN479232

Product Details anti-H-2Dd Antibody (PE)

Target: H-2Dd (MHC Class I H-2Dd)

Binding Specificity: N-Term

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H-2Dd antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)

Specificity: Reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with b2-microglobulin. Does not react with H-2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. Has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.

Purification: Purified

Immunogen: C57BL/6 x DBA/2) F1 hybrid mouse splenocytes.

Type of Immunogen: Cells

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Ctox, Flo, IP

Usage: Flow cytometry. Complement-dependent cytotoxicity. Blocking of antigen recognition. Immunoprecipitation. Immunoaffinity chromatography. Flow Cytometry: 0.2 μg/1 x10⁶. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide, stabilizing agent.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for H-2Dd

Target: H-2Dd (MHC Class I H-2Dd)

Abstract: H-2Dd Products