Keratin 5/14 antibody (C-Term)

Catalog No. ABIN472479

Product Details anti-Keratin 5/14 Antibody

Target: Keratin 5/14 (KRT5/14)

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/14 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: This antibody cocktail reacts with cytokeratin 5 intermediate filament protein as well as to 50kD cytokeratin protein designated cytokeratin 14. Basal cells of human epidermis express acidic cytokeratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58kD protein, which is closely related to cytokeratin 6. Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis and rectal-anal region. Cytokeratin 5 can be useful in the distinction of mesotheliomas from most adenocarcinomas. Cytokeratin 14 belongs to the type A (acidic) subfamily of low MW cytokeratins and exists in combination with cytokeratin 15. This antibody can be useful to distinguish stratified epithelial cells from simple epithelial cells. Species cross-reactivity: This antibody is reactive against human.

Purification: Purified

Immunogen: Prokaryotic recombinant protein corresponding to 103 amino acid portion of C-terminal region of the human cytokeratin 5 molecule. LLLLL: Synthetic peptide of the extreme c-terminal of human keratin-14.

Type of Immunogen: Synthetic peptide

Isotype: IgG1, IgG3

Application Details

Application Notes: Approved: ICC, IF, IHC, IHC-Fr

Usage: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (Fr) and Immunocytochemistry. Positive control: Skin.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.08 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Do not freeze. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing will result in a substantial loss of activity.

Target Details for Keratin 5/14

Target: Keratin 5/14 (KRT5/14)

Alternative Name: Cytokeratin 5+14 (KRT5/14 Products)

Background: This antibody detects: Cytokeratin 5+14