GQ alpha/g11 alpha (C-Term) antibody
Reactivity: Bat, Chicken, Cow, Dog, Goat, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Xenopus laevis, Zebrafish (Danio rerio)
IP, WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- GQ alpha/g11 alpha
- Binding Specificity
- C-Term
- Reactivity
- Bat, Chicken, Cow, Dog, Goat, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Xenopus laevis, Zebrafish (Danio rerio)
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Specificity
- Specific for human Gq alpha and G11 alpha. Does not recognize other non-pertussis toxin-sensitive G proteins. Species cross-reactivity: Mouse and human.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%) Water flea (90%).
- Purification
- Purified
- Immunogen
-
KLH-conjugated, synthetic peptide (QLNLKEYNLV) corresponding to amino acids 350- 359 of G11 alpha or 344-353 of Gq alpha. This sequence is highly conserved among species. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%), Water flea (90%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Isotype
- IgG
-
- Application Notes
-
Approved: IP (1:500 - 1:2000), WB
Usage: Suitable for use in Western Blot analysis and Immunoprecipitation. Immunoprecipitation: 1:500-1:2000 (detects Gq alpha and G11 alpha in 20 μg of mouse brain microsomes and membranes, detected Gq/11 a in 10 μg of mouse 3T3/NIH fibroblast soluble plasma membranes. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.1 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
-
- Target
- GQ alpha/g11 alpha
-
You are here:
