Desmoglein 1/2 antibody (C-Term)
DSG1/2
Reactivity: Cow, Human, Mouse, Rat
IF, IHC, WB, IHC (fro), IHC (p)
Host: Mouse
Monoclonal
unconjugated
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- Target See all Desmoglein 1/2 (DSG1/2) products
- Desmoglein 1/2 (DSG1/2)
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Binding Specificity
- C-Term
- Reactivity
- Cow, Human, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Desmoglein 1/2 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- Species (tested so far) Human, bovine, rat, mouse. Recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells, does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines, bovine BMGE+H, BMGE-H, MDBK.
- Purification
- Protein A purified
- Immunogen
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Band 3 polypeptide of isolated desmosomes from bovine muzzle epidermis.
Type of Immunogen: Protein - Isotype
- IgG1
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- Application Notes
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Approved: IF, IHC, IHC-Fr, IHC-P, WB
Usage: Suitable for use in Immunohistochemistry (frozen sections, paraffin-embedded tissue after microwave treatment), Western Blot, Cryo, Paraffin (Microwave), Immunofluorescence microscopy. Immunohistochemistry: 1:10 with PBS, pH 7.4, Incubation Time 1 h at RT, extended with paraffin. - Comment
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Target Species of Antibody: Bovine
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- deionized water
- Concentration
- Lot specific
- Buffer
- Lyophilized.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- Desmoglein 1/2 (DSG1/2)
- Alternative Name
- Desmoglein 1/2 (DSG1/DSG2) (DSG1/2 Products)
- Synonyms
- DSG1 antibody, desmoglein 1 antibody, DSG1 antibody
- Background
- This antibody detects: DSG1+2 / Desmoglein 1+2
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