PCDH11Y antibody (AA 918-929)

Catalog No. ABIN466846

Product Details anti-PCDH11Y Antibody

Target: PCDH11Y (Protocadherin 11 Y-Linked (PCDH11Y))

Binding Specificity: AA 918-929

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PCDH11Y antibody is un-conjugated

Application: ELISA

Specificity: Human PCDH11Y. This antibody is expected to recognize all reported Y-linked isoforms (NP_116753.1, NP_116754.1, NP_116755.1) and all reported X-linked isoforms (NP_055337.1, NP_055337.1, NP_116750.1, NP_116751.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon (100%) Gorilla, Mouse, Rat, Hamster, Bovine, Horse, Pig, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (92%) Dog (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KYNWVTTPTTFK from an internal region of human PCDH11Y (NP_116753.1, NP_116754.1, NP_116755.1, NP_055337.1, NP_055337.1, NP_116750.1, NP_116751.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon (100%), Gorilla, Mouse, Rat, Hamster, Bovine, Horse, Pig, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (92%), Dog (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA

Usage: Western Blot: Preliminary experiments gave an approx 75kD band in Human Brain (Amygdala, Hippocampus, Substantia nigra) lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115kD according to NP_116753.1 and of 113kD according to NP_055337.1, the smallest reported isoforms. The 75kD band was successfully blocked by incubation with the immunizing peptide. Have any further splice variants/modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PCDH11Y

Target: PCDH11Y (Protocadherin 11 Y-Linked (PCDH11Y))

Alternative Name: PCDH11Y / Protocadherin Y (PCDH11Y Products)

Synonyms: PCDH-PC antibody, PCDH22 antibody, PCDHY antibody, protocadherin 11 Y-linked antibody, PCDH11Y antibody

Background: Name/Gene ID: PCDH11Y

Synonyms: PCDH11Y, PCDH22, PCDHY, Protocadherin Y, Protocadherin-PC, Protocadherin prostate cancer, Protocadherin-11 Y-linked, PCDH11, Protocadherin 11 Y-linked, Protocadherin 22, Protocadherin-11, PCDH-PC, PCDH-Y, Protocadherin-22

Gene ID: 83259

NCBI Accession: NP_116753, NP_116754, NP_116755, NP_055337, NP_116750, NP_116751

UniProt: Q9BZA8