PDGF-AA Homodimer antibody (Biotin)
-
- Target
- PDGF-AA Homodimer
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Biotin
-
Application
- Western Blotting (WB), ELISA
- Specificity
- Human PDGF-AA
- Purification
- Immunoaffinity purified
- Immunogen
-
E.coli derived recombinant Human PDGF-AA.
Type of Immunogen: Cell extract
-
-
- Application Notes
-
Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hPDGF-AA by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PDGF-AA (LS-B7488) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-AA is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Sterile PBS containing 0.1 % BSA
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
- Target
- PDGF-AA Homodimer
- Alternative Name
- PDGF-AA
- Synonyms
- PDGF-A antibody, PDGF1 antibody, AA409063 antibody, CD142 antibody, Cf-3 antibody, Cf3 antibody, TF antibody, PDGFACP antibody, platelet derived growth factor subunit A antibody, coagulation factor III antibody, platelet derived growth factor, alpha antibody, PDGFA antibody, F3 antibody, Pdgfa antibody
- Background
-
Name/Gene ID: PDGFA
Family: PDGF
Synonyms: PDGFA, PDGF-A, PDGF A-chain, PDGF subunit A, PDGF-1, PDGF1 - Gene ID
- 5154
- UniProt
- P04085
-