M-CSF/CSF1 antibody (Biotin)

Catalog No. ABIN465550

Product Details anti-M-CSF/CSF1 Antibody (Biotin)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This M-CSF/CSF1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human M-CSF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human M-CSF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hM-CSF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human M-CSF (LS-C104656) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: CSF1 / CSF-1 / MCSF (CSF1 Products)

Synonyms: CSF-1 antibody, MCSF antibody, C87615 antibody, Csfm antibody, op antibody, csf1-1 antibody, zgc:172186 antibody, CSF1 antibody, csf1-2 antibody, zgc:158436 antibody, colony stimulating factor 1 antibody, colony stimulating factor 1 (macrophage) antibody, colony stimulating factor 1a (macrophage) antibody, macrophage colony-stimulating factor 1 antibody, macrophage colony stimulating factor antibody, colony stimulating factor 1b (macrophage) antibody, CSF1 antibody, Csf1 antibody, csf1a antibody, LOC396599 antibody, LOC100860895 antibody, csf1b antibody

Background: Name/Gene ID: CSF1
Family: Hormone

Synonyms: CSF1, CSF-1, MCSF, M-CSF, Lanimostim

Gene ID: 1435

UniProt: P09603

Pathways: RTK Signaling