Cardiotrophin 1 antibody
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- Target See all Cardiotrophin 1 (CTF1) Antibodies
- Cardiotrophin 1 (CTF1)
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Cardiotrophin 1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificity
- Human Cardiotrophin-1
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human Cardiotrophin-1.
Type of Immunogen: Cell extract - Top Product
- Discover our top product CTF1 Primary Antibody
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- Application Notes
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Approved: ELISA, Neut (0.25 - 0.4 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCardiotrophin-1 (15 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: To detect hCT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CT-1 (LS-C104694) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCT-1. Western Blot: To detect hCT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCT-1 is 1.5-3 ng/lane, under reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- Cardiotrophin 1 (CTF1)
- Alternative Name
- CFT1 / CT-1 / Cardiotrophin-1 (CTF1 Products)
- Synonyms
- CTF1 antibody, ct1 antibody, ct-1 antibody, ctf2 antibody, CT-1 antibody, CT1 antibody, cardiotrophin 1 antibody, cardiotrophin-1 antibody, CTF1 antibody, ctf1 antibody, Ctf1 antibody, LOC100736818 antibody
- Background
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Name/Gene ID: CTF1
Synonyms: CTF1, Cardiotrophin 1, Cardiotrophin-1, CT1, Cardiophin 1, CT-1 - Gene ID
- 1489
- UniProt
- Q16619
- Pathways
- JAK-STAT Signaling
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