Interferon gamma antibody
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- Target See all Interferon gamma (IFNG) Antibodies
- Interferon gamma (IFNG)
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Interferon gamma antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Specificity
- Rat IFN-gamma
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Rat IFN-gamma.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IFNG Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the cortex and median eminence) tissue. The primary antibody was incubated at 0.25 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. ELISA: To detect Rat IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IFN-gamma (LS-C104610) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IFN-gamma. Western Blot: To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- IFN Gamma / Interferon Gamma (IFNG Products)
- Synonyms
- IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody
- Background
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Name/Gene ID: IFNG
Family: Interferon
Synonyms: IFNG, IFG, Immune interferon, IFI, Interferon gamma, IFN-gamma, Interferon, gamma - Gene ID
- 3458
- UniProt
- P01579
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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