IL-8 antibody

Catalog No. ABIN465357

Product Details anti-IL-8 Antibody

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human IL-8

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-8.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentrations are 1 μg/mL for two hours at room temperature or 0.25 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. . Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-8 (100 ng/mL), a concentration of 2-3 μg/mL of this antibody is required. ELISA: To detect Human IL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-8 (LS-C104790) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-8. Western Blot: To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: CXCL8 (IL8 Products)

Synonyms: CXCL8 antibody, GCP-1 antibody, GCP1 antibody, LECT antibody, LUCT antibody, LYNAP antibody, MDNCF antibody, MONAP antibody, NAF antibody, NAP-1 antibody, NAP1 antibody, IL-8 antibody, AMCF-I antibody, il8 antibody, IL8 antibody, si:dkey-151b16.2 antibody, cxcli2 antibody, cxcl8 antibody, gcp-1 antibody, il-8 antibody, lynap antibody, mdncf antibody, monap antibody, nap-1 antibody, C-X-C motif chemokine ligand 8 antibody, interleukin 8 antibody, chemokine (C-X-C motif) ligand 8a antibody, chemokine (C-X-C motif) ligand 1-like antibody, chemokine (C-X-C motif) ligand 8 L homeolog antibody, CXCL8 antibody, il8 antibody, cxcl8a antibody, IL8 antibody, Cxcl8 antibody, LOC422654 antibody, cxcl8.L antibody

Background: Name/Gene ID: CXCL8
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL8, 3-10C, AMCF-I, B-ENAP, C-X-C motif chemokine 8, GCP-1, IL-8, IL8, Interleukin-8, K60, LECT, GCP1, LUCT, NAF, NAP1, Neutrophil-activating factor, Interleukin 8, Protein 3-10C, MDNCF, TSG-1, Emoctakin, LYNAP, MONAP, NAP-1, SCYB8, T-cell chemotactic factor

Gene ID: 3576

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy