IL12 antibody

Catalog No. ABIN465114

Product Details anti-IL12 Antibody

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IL12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Mouse IL-12

Purification: Immunoaffinity purified

Immunogen: CHO cell derived recombinant Murine IL-12.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (2 - 3 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-12 (2 ng/mL), a concentration of 002-003 μg/mL of this antibody is required. ELISA: To detect mIL-12 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-12 (LS-C104409) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-12. Western Blot: To detect mIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-12 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL12 / IL-12 (IL12 Products)

Synonyms: CLMF antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12 antibody, LOC100217488 antibody, IL-12p35 antibody, Il-12a antibody, Ll12a antibody, p35 antibody, IL12p40 antibody, IL12B antibody, IL-12 p35 antibody, interleukin 12B antibody, interleukin 12A antibody, interleukin 12b antibody, interleukin 12a antibody, Interleukin 12 level antibody, IL12B antibody, IL12A antibody, Il12b antibody, Il12a antibody, IL12 antibody, il12a antibody

Background: Name/Gene ID: IL-12

Synonyms: IL-12

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome