TWEAK antibody

Catalog No. ABIN465064

Product Details anti-TWEAK Antibody

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TWEAK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human TWEAK

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TWEAK.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 - 0.2 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTWEAK (200 ng/mL), a concentration of 0.1-0.2 μg/mL of this antibody is required. ELISA: To detect hTWEAK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TWEAK (LS-C104282) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK. Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTWEAK is 1.5-3 ng/lane, under either reducing or or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TWEAK

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Alternative Name: TNFSF12 / TWEAK (TNFSF12 Products)

Synonyms: APO3L antibody, DR3LG antibody, TWEAK antibody, Apo3l antibody, Dr3l antibody, Dr3lg antibody, Tweak antibody, TNF superfamily member 12 antibody, tumor necrosis factor (ligand) superfamily, member 12 antibody, TNFSF12 antibody, Tnfsf12 antibody

Background: Name/Gene ID: TNFSF12
Family: TNF

Synonyms: TNFSF12, APO3/DR3 ligand, APO3L, DR3LG, APO3 ligand, TWEAK

Gene ID: 8742

UniProt: O43508

Pathways: Apoptosis