IL-11 antibody (Biotin)

Catalog No. ABIN465008

Product Details anti-IL-11 Antibody (Biotin)

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-11 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human IL-11

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-11.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hIL-11 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-11. To detect hIL-11 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-11 (LS-C104366) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-11. Western Blot: To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-11

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Alternative Name: IL11 (IL11 Products)

Synonyms: IL11 antibody, AGIF antibody, IL-11 antibody, Il-11 antibody, interleukin 11 antibody, IL11 antibody, Il11 antibody

Background: Name/Gene ID: IL11
Family: Hormone

Synonyms: IL11, AGIF, Adipogenesis inhibitory factor, IL-11, Interleukin-11, Oprelvekin, Interleukin 11

Gene ID: 3589

UniProt: P20809

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion