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CCL15 antibody

CCL15 Reactivity: Human ELISA, WB, Neut Host: Goat Polyclonal unconjugated
Catalog No. ABIN464833
  • Target See all CCL15 Antibodies
    CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))
    Reactivity
    Human
    Host
    • 36
    • 5
    • 5
    • 1
    • 1
    Goat
    Clonality
    • 44
    • 4
    Polyclonal
    Conjugate
    • 22
    • 8
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CCL15 antibody is un-conjugated
    Application
    • 33
    • 28
    • 21
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Neutralization (Neut)
    Specificity
    Human MIP-5
    Purification
    Immunoaffinity purified
    Immunogen
    E.coli derived recombinant Human MIP-5.

    Type of Immunogen: Cell extract
  • Application Notes
    Approved: ELISA (0.5 - 2 μg/mL), Neut (1.75 - 3 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIP-5 (50 ng/mL), a concentration of 1.75-3 μg/mL of this antibody is required. ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-5 (LS-C104743) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-5. Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Sterile water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS,  pH 7.2
    Handling Advice
    Avoid freeze/thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • Target
    CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))
    Alternative Name
    CCL15 / MIP5 (CCL15 Products)
    Synonyms
    HCC-2 antibody, HMRP-2B antibody, LKN-1 antibody, LKN1 antibody, MIP-1 delta antibody, MIP-1D antibody, MIP-5 antibody, MRP-2B antibody, NCC-3 antibody, NCC3 antibody, SCYA15 antibody, SCYL3 antibody, SY15 antibody, C-C motif chemokine ligand 15 antibody, CCL15 antibody
    Background
    Name/Gene ID: CCL15
    Subfamily: Intercrine beta
    Family: Intercrine

    Synonyms: CCL15, C-C motif chemokine 15, Chemokine hcc2, HMRP-2B, Leukotactin-1, LKN1, MIP-5, MIP5, MRP-2B, Leukotactin1, Small-inducible cytokine A15, MIP-1D, NCC3, New CC chemokine 3, SY15, Chemokine CC-2, HCC-2, Leukotactin 1, LKN-1, MIP-1 delta, NCC-3, SCYA15
    Gene ID
    6359
    UniProt
    Q16663
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