CAMK2B antibody
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- Target See all CAMK2B Antibodies
- CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))
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Reactivity
- Human, Mouse, Rat, Cow, Horse, Rabbit, Zebrafish (Danio rerio), Dog, Monkey, Xenopus laevis, Chicken, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CAMK2B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%) Orangutan (93%).
- Purification
- Immunoaffinity purified
- Immunogen
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A 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (residues 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%), Orangutan (93%).
Type of Immunogen: Synthetic peptide - KLH conjugated - Top Product
- Discover our top product CAMK2B Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- BBS, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
- Storage
- -20 °C
- Storage Comment
- Store at -20°C.
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- Target
- CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))
- Alternative Name
- CAMK2 / CAMKII (CAMK2B Products)
- Synonyms
- CAM2 antibody, CAMK2 antibody, CAMKB antibody, Camk2d antibody, Ck2b antibody, camk2 antibody, camk2b antibody, cam2 antibody, camkb antibody, CAMK2B antibody, calcium/calmodulin dependent protein kinase II beta antibody, calcium/calmodulin-dependent protein kinase II, beta antibody, calcium/calmodulin-dependent protein kinase II beta antibody, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antibody, calcium/calmodulin dependent protein kinase (CaM kinase) II beta L homeolog antibody, calcium/calmodulin-dependent protein kinase (CaM kinase) II beta antibody, CAMK2B antibody, Camk2b antibody, camk2a.S antibody, camk2b.L antibody
- Background
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Name/Gene ID: CAMK2
Synonyms: CAMK2 - Pathways
- WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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