S100A9 antibody (AA 67-79)
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- Target See all S100A9 Antibodies
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
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Binding Specificity
- AA 67-79
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This S100A9 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human S100A9.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Hamster, Bovine, Rabbit, Horse, Pig (92%) Panda (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-DTNADKQLSFEEF from an internal region of human S100A9 (NP_002956.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Hamster, Bovine, Rabbit, Horse, Pig (92%), Panda (85%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product S100A9 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 16kD band observed in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kD according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kD) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16,280(50):41521-9., PMID: 16216873). Recommended concentration: 0.3-1 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- S100A9 (S100 Calcium Binding Protein A9 (S100A9))
- Alternative Name
- S100A9 / MRP14 (S100A9 Products)
- Synonyms
- 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, S100A9 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody
- Background
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Name/Gene ID: S100A9
Synonyms: S100A9, 60B8AG, Calgranulin B, CFAG, CAGB, Calgranulin-B, Calprotectin L1H subunit, CGLB, L1Ag, MRP14, NIF, p14, Protein S100-A9, LIAG, MAC387, MRP-14 - Gene ID
- 6280
- NCBI Accession
- NP_002956
- UniProt
- P06702
- Pathways
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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