Glucose-6-Phosphate Dehydrogenase antibody (AA 35-506)

Catalog No. ABIN462273

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Binding Specificity: AA 35-506

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human G6PD (35-506aa)

Purification: Protein G purified

Immunogen: Recombinant human G6PD (35-506aa) purified from E. coli

Type of Immunogen: Recombinant protein

Clone: AT2F6

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD (G6PD Products)

Background: Name/Gene ID: G6PD

Synonyms: G6PD, G6PD1

Gene ID: 2539

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones