Ubiquitin antibody

Catalog No. ABIN462097

Product Details anti-Ubiquitin Antibody

Target: Ubiquitin

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog, Chicken, Monkey, Sheep, Drosophila melanogaster, Guinea Pig, Hamster, Fish, Saccharomyces cerevisiae, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Purification: Protein A purified

Immunogen: Native bovine ubiquitin, conjugated to KLH.

Type of Immunogen: Native protein

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for Ubiquitin

Target: Ubiquitin

Alternative Name: Ubiquitin (Ubiquitin Products)

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway