BRAP antibody (AA 67-79)

Catalog No. ABIN462090

Product Details anti-BRAP Antibody

Target: BRAP (BRCA1 Associated Protein (BRAP))

Binding Specificity: AA 67-79

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAP antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: KLH conjugated synthetic peptide comprising amino acids 27 - 39 [AAGEMSDEEIKKT] of the human BRCA1 associated protein (BRAP) [N-terminus] protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Elephant, Panda, Dog, Horse (92%) Hamster, Rabbit, Pig (85%).

Purification: Protein G purified

Immunogen: Synthetic peptide from human BRAP. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Elephant, Panda, Dog, Horse (92%), Hamster, Rabbit, Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:1000), IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BRAP

Target: BRAP (BRCA1 Associated Protein (BRAP))

Alternative Name: BRAP2 / IMP (BRAP Products)

Background: Name/Gene ID: BRAP

Synonyms: BRAP, BRAP2, IMP, Galectin-2-binding protein, RING finger protein 52, RNF52, BRCA1 associated protein, BRCA1-associated protein

Gene ID: 8315

UniProt: Q7Z569