Ubiquitin antibody

Catalog No. ABIN462018

Product Details anti-Ubiquitin Antibody

Target: Ubiquitin

Reactivity: Human, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.

Purification: Protein A purified

Immunogen: Purified bovine ubiquitin conjugated to carrier protein.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL, Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl,  pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Ubiquitin

Target: Ubiquitin

Alternative Name: Ubiquitin (Ubiquitin Products)

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway