FLIP antibody (AA 191-209)

Catalog No. ABIN461877

Product Details anti-FLIP Antibody

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Binding Specificity: AA 191-209

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-P (5 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kD and 25 kD bands can be detected. The antibody recognizes human and murine FLIPg and FLIPd but not FLIPa and FLIPb.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR / FLIP (CFLAR Products)

Background: Name/Gene ID: CFLAR
Subfamily: Cysteine C14
Family: Apoptosis

Synonyms: CFLAR, C-FLIPR, C-FLIPS, C-FLIP, CASP8AP1, Cflip, CLARP, CASH, Caspase homolog, Caspase-eight-related protein, Flice inhibitory protein, I-FLICE, Inhibitor of FLICE, FLAME, FLAME1, MRIT, Usurpin beta, C-FLIPL, Casper, FLAME-1, FLIP, Usurpin

Gene ID: 8837

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development