Pyruvate Oxidase antibody
POX
Reactivity: Bacteria
ELISA, WB, IF
Host: Rabbit
Polyclonal
unconjugated
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- Target See all Pyruvate Oxidase (POX) Antibodies
- Pyruvate Oxidase (POX)
- Reactivity
- Bacteria
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Pyruvate Oxidase antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Characteristics
- IgG fraction of polyclonal rabbit antiserum to pyruvate oxidase from bacteria
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- Pyruvate oxidase isolated and purified from bacteria. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. No foreign proteins added.
- Buffer
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Target
- Pyruvate Oxidase (POX)
- Alternative Name
- Pyruvate Oxidase (POX Products)
- Synonyms
- pyruvate oxidase antibody, thiamine pyrophosphate-containing protein YdaP antibody, hypothetical protein, similar to pyruvate oxidase antibody, similar to pyruvate oxidase antibody, SP_0730 antibody, spxB antibody, ydaP antibody, lmo0722 antibody, MW2460 antibody, pox1 antibody, pox5 antibody, SA2327 antibody, lin0730 antibody, PsycPRwf_1595 antibody, pox antibody
- Background
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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