PLC antibody
PLC
Reactivity: Bacillus cereus
WB, IF, ELISA
Host: Rabbit
Polyclonal
unconjugated
-
- Target See all PLC Antibodies
- PLC (Phospholipase C (PLC))
-
Reactivity
- Bacillus cereus
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This PLC antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), ELISA
- Specificity
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Characteristics
- IgG fraction of polyclonal rabbit antiserum to phospholipase C from Bacillus cereus
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- Phospholipase C isolated and purified from Bacillus cereus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
-
-
- Application Notes
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. No foreign proteins added.
- Buffer
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
- Target
- PLC (Phospholipase C (PLC))
- Alternative Name
- Phospholipase C (PLC Products)
- Synonyms
- GB16555 antibody, PLC antibody, ARABIDOPSIS THALIANA PHOSPHOLIPASE C antibody, ATPLC antibody, ATPLC1 antibody, MZN1.12 antibody, MZN1_12 antibody, PHOSPHOLIPASE C antibody, phospholipase C 1 antibody, phospholipase C1 antibody, BA0677 antibody, BCE0744 antibody, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 antibody, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II antibody, phospholipase C antibody, phospholipase C1 antibody, uncharacterized LOC100851276 antibody, plc-1 antibody, LOC408791 antibody, PLC antibody, PLC1 antibody, plc antibody, plC antibody, LOC100851276 antibody, LOC100009319 antibody
- Background
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
- Pathways
- TCR Signaling, Response to Water Deprivation, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Phototransduction
-
You are here:
