NADH Peroxidase antibody

Catalog No. ABIN459237

Product Details anti-NADH Peroxidase Antibody

Target: NADH Peroxidase

Reactivity: Enterococcus faecalis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NADH Peroxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: IgG fraction of polyclonal rabbit antiserum to NADH peroxidase from Streptococcus faecalis

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: NADH peroxidase isolated and purified from Streptococcus faecalis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for NADH Peroxidase

Target: NADH Peroxidase

Abstract: NADH Peroxidase Products

Synonyms: putative NADH peroxidase antibody, NADH peroxidase antibody, NAD(FAD)-dependent dehydrogenase antibody, SPy_1681 antibody, spyM18_1693 antibody, npx antibody, SPs0401 antibody, npr antibody, MA_RS07405 antibody, M6_Spy1426 antibody, M5005_Spy_1378 antibody, M28_Spy1421 antibody, MBAR_RS14975 antibody, LSL_0137 antibody, MBUR_RS05050 antibody, SpyM50412 antibody, LCRIS_RS02695 antibody, Lbuc_1777 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.