Pyrophosphatase (Inorganic) 1 (PPA1) antibody

Catalog No. ABIN459215

Product Details

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: IgG fraction of polyclonal rabbit antiserum to inorganic pyrophosphatase from yeast

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Inorganic pyrophosphatase is isolated and purified from yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

References

Patir-Nebioglu, Andrés, Krebs, Fink, Drzewicka, Stankovic-Valentin, Segami, Schuck, Büttner, Hell, Maeshima, Melchior, Schumacher: "Pyrophosphate modulates plant stress responses via SUMOylation." in: eLife, Vol. 8, (2019) (PubMed).

Target Details

Target: Pyrophosphatase (Inorganic) 1 (PPA1)

Alternative Name: Inorganic Pyrophosphatase (PPA1 Products)

Synonyms: ppa antibody, SCE9.16 antibody, PSPTO0722 antibody, AO090005001437 antibody, IOPPP antibody, PP antibody, PP1 antibody, SID6-8061 antibody, 2010317E03Rik antibody, Pyp antibody, Pp antibody, inorganic pyrophosphatase antibody, Inorganic pyrophosphatase antibody, pyrophosphatase (inorganic) 1 L homeolog antibody, pyrophosphatase (inorganic) 1 antibody, ppa antibody, SCO3409 antibody, ppa-1 antibody, APE_1692.1 antibody, AOR_1_2492174 antibody, Celal_2154 antibody, Celly_1958 antibody, Dester_1338 antibody, Marky_0054 antibody, Halhy_5834 antibody, ppa1.L antibody, PPA1 antibody, Ppa1 antibody

Target Type: Viral Protein

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.