Glucose Oxidase antibody
-
- Target See all Glucose Oxidase (GOD) products
- Glucose Oxidase (GOD)
-
Reactivity
- Aspergillus niger
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Glucose Oxidase antibody is un-conjugated
-
Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Characteristics
- IgG fraction of polyclonal rabbit antiserum to glucose oxidase from Aspergillus niger
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- Glucose oxidase isolated and purified from Aspergillus niger. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
-
-
- Application Notes
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. No foreign proteins added.
- Buffer
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
- Target
- Glucose Oxidase (GOD)
- Alternative Name
- Glucose Oxidase (GOD Products)
- Synonyms
- LOC406081 antibody, GB19418 antibody, glucose oxidase antibody, LOC406081 antibody, AFUA_2G14840 antibody, SS1G_10949 antibody, AOR_1_952194 antibody, AOR_1_786174 antibody, AOR_1_358034 antibody, CC1G_15386 antibody, VDBG_09040 antibody, VDBG_06502 antibody, SCHCODRAFT_113971 antibody
- Background
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
-