Collagenase antibody

Catalog No. ABIN459188

Product Details anti-Collagenase Antibody

Target: Collagenase

Reactivity: Clostridium histolyticum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagenase antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: IgG fraction of polyclonal rabbit antiserum to collagenase from Clostridium histolyticum

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: Collagenase is isolated and purified from Clostridium histolyticum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 10mg lyof. in 1ml PBS

Buffer: Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details for Collagenase

Target: Collagenase

Abstract: Collagenase Products

Synonyms: collagenase antibody, RB3913 antibody, BPSS0666 antibody, Sbal_3732 antibody, Bcer98_0486 antibody, Shew185_0630 antibody, Shal_3056 antibody, Sbal195_0657 antibody, Lbys_3550 antibody, Palpr_2084 antibody

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.