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PFKM antibody (Biotin)

PFKM Reactivity: Rabbit WB, ELISA, IF Host: Sheep Polyclonal Biotin
Catalog No. ABIN459123
  • Target See all PFKM Antibodies
    PFKM (phosphofructokinase, Muscle (PFKM))
    Reactivity
    • 67
    • 28
    • 23
    • 13
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 59
    • 9
    • 7
    • 7
    Sheep
    Clonality
    • 67
    • 15
    Polyclonal
    Conjugate
    • 47
    • 10
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This PFKM antibody is conjugated to Biotin
    Application
    • 63
    • 36
    • 29
    • 13
    • 12
    • 8
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Specificity
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    Characteristics
    Biotin-conjugated IgG fraction of polyclonal sheep antiserum to fructose-6-phosphate kinase from rabbit muscle
    Purification
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    Immunogen
    Fructose-6-phosphate kinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
    Top Product
    Discover our top product PFKM Primary Antibody
  • Application Notes
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.5. No foreign proteins added.
    Buffer
    Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    PFKM (phosphofructokinase, Muscle (PFKM))
    Alternative Name
    Fructose-6-phosphate Kinase (PFKM Products)
    Synonyms
    GSD7 antibody, PFK-1 antibody, PFK1 antibody, PFKA antibody, PFKX antibody, AI131669 antibody, PFK-A antibody, PFK-M antibody, Pfk-4 antibody, Pfk4 antibody, Pfka antibody, Pfkx antibody, Pfk-M antibody, M-PFK antibody, PFKM antibody, pfkm antibody, zgc:92344 antibody, pfk antibody, im:7137110 antibody, wu:fc58g11 antibody, phosphofructokinase, muscle antibody, phosphofructokinase, platelet antibody, phosphofructokinase, muscle a antibody, phosphofructokinase, muscle S homeolog antibody, phosphofructokinase, muscle b antibody, PFKM antibody, Pfkm antibody, PFKP antibody, pfkma antibody, pfkm.S antibody, pfkmb antibody
    Background
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect
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