RNA Polymerase antibody (Biotin)
Reactivity: E. coli
WB, IF, ELISA
Host: Rabbit
Polyclonal
Biotin
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- Target See all RNA Polymerase products
- RNA Polymerase
- Reactivity
- E. coli
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RNA Polymerase antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunofluorescence (IF), ELISA
- Specificity
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- Characteristics
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to ribonucleic acid polymerase from Escherichia coli
- Purification
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- Immunogen
- Ribonucleic acid polymerase isolated and purified from Escherichia coli. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.9. No foreign proteins added.
- Buffer
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Target
- RNA Polymerase
- Alternative Name
- Ribonucleic Acid Polymerase (RNA Polymerase Products)
- Synonyms
- RNA polymerase antibody, polymerase antibody
- Background
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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